Whole Exome Sequencing (WES)
For DNA application, we provide targeted sequencing with optional basic data analysis.
DNA is sheared enzymatically or mechanically into size distribution appropriate for individual assays before end repair, ligation of adaptors and amplification to construct libraries for sequencing. With targeted sequencing, the sequences of interest will be pulled out from the libraries using Agilent Technologies SureSelect Target Enrichment products before sequencing. SureSelect uses probe-based hybridisation for capturing sequences of interest. Unique indexes are added to individual samples for multiplexing on the sequencer.
Whole Genome Sequencing (WGS)
For DNA application, we provide whole genome sequencing with optional basic data analysis.
DNA is sheared enzymatically or mechanically into size distribution appropriate for individual assays before end repair, ligation of adaptors and amplification to construct libraries for sequencing.
Target Enrichment
Target enrichment probes are available for a range of model organisms. Probes can be customized for any species whose genome is available in RefSeq using Agilent Technologies SureDesign portal. Please check with us if your species of choice is applicable.
Strand-specific RNA-seq / Whole Transcriptome Sequencing
For RNA application, we provide strand specific whole transcriptome, mRNA, or targeted RNA sequencing with optional basic data analysis.
Double stranded DNA is synthesized from the RNA before fragmentation, end repair, ligation of adaptors and amplification to construct libraries for sequencing. For targeted RNA sequencing, the sequences of interest will be pulled out from the libraries using Agilent Technologies SureSelect Target Enrichment products before sequencing. SureSelect uses probe-based hybridisation for capturing sequences of interest. Unique indexes are added to individual samples for multiplexing on the sequencer.
Target enrichment probes are available for a range of model organisms. Probes can be customized for any species whose genome is available in RefSeq using Agilent Technologies SureDesign portal. Please check with us if your species of choice is applicable.
For nucleic acid quality, the general guideline is, RNA should have absorbance ratios of 260/280 ≥ 2, 260/230 ≥ 2 and RIN ≥ 7. Intact genomic DNA should have absorbance ratios of 260/280 ≥ 1.8, 260/230 ≥ 2 and appear as a single high molecular weight band on electrophoresis. Please discuss with us on your sample quality as the guidelines may not be applicable for your sample types/conditions.
Methyl-seq Service
Methylation is responsible for a vast range of biological functions such as regulating tissue-specific gene expression, genomic imprinting, and X chromosome inactivation. We provide Methyl-Seq services using SureSelect target enrichment to investigate the methylation of CpG islands in addition to whole genome bisulfite sequencing. We also provide ChIP-seq Service. Please feel free to reach out to us to discuss the applicability of this service for you.
Bioinformatics and Data Analysis Services
We provide data analysis services for NGS data. For RNA-seq data, our services include, trimming, alignment to reference genome and identification of differentially expressed genes between conditions. For DNA-seq data, we provide analysis of Agilent Target Enrichment dataset using Surecall for reference genome alignment and mutation screening. Our analysis service is customizable and please speak to discuss how we may help you address your requirements.
For DNA application, we provide targeted sequencing with optional basic data analysis.
DNA is sheared enzymatically or mechanically into size distribution appropriate for individual assays before end repair, ligation of adaptors and amplification to construct libraries for sequencing. With targeted sequencing, the sequences of interest will be pulled out from the libraries using Agilent Technologies SureSelect Target Enrichment products before sequencing. SureSelect uses probe-based hybridisation for capturing sequences of interest. Unique indexes are added to individual samples for multiplexing on the sequencer.
For DNA application, we provide whole genome sequencing with optional basic data analysis.
DNA is sheared enzymatically or mechanically into size distribution appropriate for individual assays before end repair, ligation of adaptors and amplification to construct libraries for sequencing.
Target enrichment probes are available for a range of model organisms. Probes can be customized for any species whose genome is available in RefSeq using Agilent Technologies SureDesign portal. Please check with us if your species of choice is applicable.
For RNA application, we provide strand specific whole transcriptome, mRNA, or targeted RNA sequencing with optional basic data analysis.
Double stranded DNA is synthesized from the RNA before fragmentation, end repair, ligation of adaptors and amplification to construct libraries for sequencing. For targeted RNA sequencing, the sequences of interest will be pulled out from the libraries using Agilent Technologies SureSelect Target Enrichment products before sequencing. SureSelect uses probe-based hybridisation for capturing sequences of interest. Unique indexes are added to individual samples for multiplexing on the sequencer.
Target enrichment probes are available for a range of model organisms. Probes can be customized for any species whose genome is available in RefSeq using Agilent Technologies SureDesign portal. Please check with us if your species of choice is applicable.
For nucleic acid quality, the general guideline is, RNA should have absorbance ratios of 260/280 ≥ 2, 260/230 ≥ 2 and RIN ≥ 7. Intact genomic DNA should have absorbance ratios of 260/280 ≥ 1.8, 260/230 ≥ 2 and appear as a single high molecular weight band on electrophoresis. Please discuss with us on your sample quality as the guidelines may not be applicable for your sample types/conditions.
Methylation is responsible for a vast range of biological functions such as regulating tissue-specific gene expression, genomic imprinting, and X chromosome inactivation. We provide Methyl-Seq services using SureSelect target enrichment to investigate the methylation of CpG islands in addition to whole genome bisulfite sequencing. We also provide ChIP-seq Service. Please feel free to reach out to us to discuss the applicability of this service for you.
We provide data analysis services for NGS data. For RNA-seq data, our services include, trimming, alignment to reference genome and identification of differentially expressed genes between conditions. For DNA-seq data, we provide analysis of Agilent Target Enrichment dataset using Surecall for reference genome alignment and mutation screening. Our analysis service is customizable and please speak to discuss how we may help you address your requirements.
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